The US$2.4 billion property opened with 110,000 sqm of dedicated convention and exhibition space, the 15,000-seat Venetian Arena and 3,000 hotel suites.
This makes the Venetian the largest hotel in Asia and also one of the largest buildings in the world.
Sheldon Adelson, chairman and chief executive officer of Las Vegas Sands Corporation, the parent company of the Venetian, said: “The Venetian Macau represents a massive shift for Macau and the future of tourism development in Asia.
"It's the first time that anybody has had the vision to develop the most mature integrated resort complex in the world."
The black-tie opening ceremony brought together some of the best-known names in Asian and global entertainment, including Cirque du Soleil.
The Venetian intends to make world-class entertainment a cornerstone of its Macau business, which will provide new opportunities for regional incentive planners.
Development is already underway on an extra 100,000 sqm of convention space and 17,000 more hotel rooms, from brands including Four Seasons, Sheraton, St. Regis, Shangri-La, Traders, Hilton, Conrad, Fairmont and Raffles.